WCSE

Raman Scattering Spectra Characterize the Influence of LED Irradiation on Human Skin Fibroblasts

Abstract— LED phototherapy has been widely applied in clinical practice for treating the skin-related diseases such as acne, pimples, burn and etc.. However, the underlying mechanism for the therapeutic effects involved in bioprocess was still unclear. Although quite a few researches proposed various potential hypotheses through strict experiments, the methods they performed with like morphological observation and immunohistochemistry seemed subjective and cumbersome operations. Raman scattering, a technique to parse molecular information by providing fingerprint spectra at the molecular level, could be a better alternative method for its high efficient and usability. Therefore, we tried to study the influences of LED light irradiation on human skin fibroblasts by comparing the Raman spectra from the cells under irradiation with three lights at different wavelengths: red (λ=625±5 nm), yellow (λ=590±5 nm) and blue (λ=465±5 nm). Results indicated red and yellow light at 625 nm and 590 nm might promote the anabolism including the synthesis of bio-macromolecules such as proteins and DNAs, while blue light at 465 nm might tend to make the macromolecules catabolized into small molecules. Raman scattering could be applied as a high efficient technique for cell researches.

Index Terms— LED, phototherapy, Raman scattering, human skin fibroblast

Zhenzhen Feng, Heng Zhang, Xiangfei Yang, Shupeng Liu, Zhenyi Chen, Na Chen, Linshui Hu
Key Laboratory of Specialty Fiber Optics and Optical Access Networks, School of Communication and Information Engineering, Shanghai University, CHINA
Taihao Li
Beijing Advanced Innovation Center for Imaging Technology, Capital Normal University, CHINA
Shupeng Liu, Linshui Hu
Zhejiang Conba, Pharmaceutical Co Ltd, CHINA

ISBN: 978-981-11-3671-9 DOI: 10.18178/wcse.2017.06.17Xsrc="http://www.wcse.org/uploadfile/2019/0823/20190823055609629.png" style="width: 120px; height: 68px;" />[Download]

Cite: Zhenzhen Feng, Heng Zhang, Xiangfei Yang, Shupeng Liu, Zhenyi Chen, Na Chen, Linshui Hu, Taihao Li, "Raman Scattering Spectra Characterize the Influence of LED Irradiation on Human Skin Fibroblasts," Proceedings of 2017 the 7th International Workshop on Computer Science and Engineering, pp. 1362-1367, Beijing, 25-27 June, 2017.